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HNRNPM

ANTIBODY INFORMATION
 
 

Antibody HPA024344

  

Antibody CAB016113

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA024344   sc-20002  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 5   4  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA024344

  

Antibody CAB016113

  
Immunocytochemistry


Validated
   Approved  
Immunohistochemistry

Supported   Supported  
Western blot


Validated
   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA024344

  

Antibody CAB016113

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		    

 

Relative fluorescence intensity (RFI)

    

Figure description

 Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:28    
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:20   1:2000  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA024344

  

Antibody CAB016113

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human pancreas shows strong nuclear positivity.   Immunohistochemical staining of human pancreas shows strong nuclear positivity.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:800   1:3500  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA024344

  

Antibody CAB016113

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 77.6, 77.5, 73.6, 39.9    

Loading control

 Total protein image    

Antibody dilution

 1:63    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 77.6, 77.5, 73.6, 39.9   77.6, 77.5, 73.6, 39.9, 38.2, 37.7, 30.1, 24.1, 22.6, 12.3, 10  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA024344

  

Antibody CAB016113

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA024344

  

Antibody CAB016113

  

Antigen

 Recombinant protein fragment   Not known  

Length (aa)

 105    

Antigen sequence

 
NKMGGMEGPFGGGMENMGRFGSGMNMGRINEILSNALKRGEIIAKQGGGG
GGGSVPGIERMGPGIDRLGGAGMERMGAGLGHGMDRVGSEIERMGLVMDR
MGSVE
   

Matching transcripts

 HNRNPM-001 - ENSP00000325732 [100%]
HNRNPM-002 - ENSP00000325376 [100%]
HNRNPM-201 - ENSP00000483715 [100%]
HNRNPM-007 - ENSP00000470635 [83%]
   

Other gene match

    
ANTIGEN VIEW
HNRNPM-001
HNRNPM-002
HNRNPM-007
HNRNPM-201

RELEVANT PUBLICATIONS
 
 

Antibody HPA024344

  

Antibody CAB016113

  

1.

 Magnetic fractionation and proteomic dissection of cellular organelles occupied by the late replication complexes of Semliki Forest virus
Varjak M et al
J Virol 2013;87(18):10295-312
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.