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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus, nucleoli & nuclear bodies.
Antibody dilution
1:6
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA021875
Antibody HPA053989
Antibody CAB037253
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human gall bladder shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 67 cell types
RNA: detected in 37 tissues Protein: detected in 71 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021875
Antibody HPA053989
Antibody CAB037253
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413712)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
51.9, 46.7
51.9
51.9, 46.7, 19.4, 17.7, 14.4, 8.2, 8
Antibody dilution
1:250
1:80
1:500
PROTEIN ARRAY
Antibody HPA021875
Antibody HPA053989
Antibody CAB037253
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:500
ANTIGEN INFORMATION
Antibody HPA021875
Antibody HPA053989
Antibody CAB037253
Antigen
Recombinant protein fragment
Recombinant protein fragment
Synthetic peptide
Length (aa)
102
48
Antigen sequence
ELQGFHRSFKGQNPFELAFSLDQPDHGDSDFGLQCSARPDMPASQPIDIP
DAKKRGKKKKRGRATDSFSGRFEDVYQLQEDVLGEGAHARVQTCINLITS
QE
