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Immunofluorescent staining of human cell line Hep G2 shows localization to vesicles.
Antibody dilution
1:72
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA055767
Antibody CAB008639
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human colon shows distinctly stained plasma.
Immunohistochemical staining of human lung shows distinct positivity in plasma.
Expression
RNA: detected in 9 tissues Protein: detected in 15 cell types
RNA: detected in 9 tissues Protein: detected in 3 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA055767
Antibody CAB008639
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
57.1
57.1
Antibody dilution
1:360
1:500
PROTEIN ARRAY
Antibody HPA055767
Antibody CAB008639
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
