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Immunofluorescent staining of human cell line A-431 shows localization to nuclear membrane.
Antibody dilution
1:118
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA001209
Standard validation
Supported
Figure description
Immunohistochemical staining of human heart muscle shows strong nuclear membrane positivity in myocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
Antibody dilution
1:1000
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001209
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
82.5, 80.3
Antibody dilution
1:500
PROTEIN ARRAY
Antibody HPA001209
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
ANTIGEN INFORMATION
Antibody HPA001209
Antigen
Recombinant protein fragment
Length (aa)
151
Antigen sequence
LKSEWQSMTQESFQESSVKELRRLEDQLAGLQQELAALALKQSSVAEEVG
LLPQQIQAVRDDVESQFPAWISQFLARGGGGRVGLLQREEMQAQLRELES
KILTHVAEMQGKSAREAAASLSLTLQKEGVIGVTEEQVHHIVKQALQRYS
E
