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Immunohistochemical staining of human kidney shows moderate cytoplasmic and nuclear positivity in renal tubules.
Immunohistochemical staining of human kidney shows distinct cytoplasmic and membranous positivity in cells in tubular cells.
Expression
RNA: detected in 4 tissues Protein: detected in 5 cell types
RNA: detected in 4 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:2000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039451
Antibody HPA039562
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413679)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413679)
Target mass (kDa)
33, 30.8
33, 30.8, 30.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039451
Antibody HPA039562
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
