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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus, cytosol & focal adhesion sites.
Antibody dilution
1:50
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA001439
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001439
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17.1 Lane 2: RT4 Lane 3: EFO-21 Lane 4: A-431 Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
86.4, 77.4
Antibody dilution
1:500
PROTEIN ARRAY
Antibody HPA001439
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:1000
ANTIGEN INFORMATION
Antibody HPA001439
Antigen
Recombinant protein fragment
Length (aa)
151
Antigen sequence
YSVVVEEVPLQPGESLPYHSVYYEDEYDDTYDGNQVGANDADSDDELISR
RPFTIPQVLRTKVPREGQEEDDDDEEDDADEEAPKPDHFVQDPAVLREKA
EARRMAFLAKKGYRHDSSTAVAGSPRGHGQSRETTQERRKKEANKATRAN
H
