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PPP4R3A

ANTIBODY INFORMATION
 
 

Antibody HPA002568

  

Antibody HPA063917

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA002568   HPA063917  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 2   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA002568

  

Antibody HPA063917

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Supported   Supported  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002568

  

Antibody HPA063917

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA063917.		  
Validated

Antibody staining overlaps with antibody HPA002568.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.  

Antibody dilution

 1:167   1:137  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002568

  

Antibody HPA063917

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in cells of seminiferus ducts.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 79 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:500    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA002568

  

Antibody HPA063917

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 95.4, 93.9, 67.8   95.4, 93.9, 67.8  

Antibody dilution

 1:250   1:460  

PROTEIN ARRAY
 
 

Antibody HPA002568

  

Antibody HPA063917

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:5500  

ANTIGEN INFORMATION
 
 

Antibody HPA002568

  

Antibody HPA063917

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 128   51  

Antigen sequence

 
DGEAVVSPSDKTKNDDDIMDPISKFMERKKLKESEEKEVLLKTNLSGRQS
PSFKLSLSSGTKTNLTSQSSTTNLPGSPGSPGSPGSPGSPGSVPKNTSQT
AAITTKGGLVGLVDYPDDDEDDDEDEDK
  
KLRFEQQRERQDNPKLDSMRSILRNHRYRRDARTLEDEEEMWFNTDEDDM
E
 

Matching transcripts

 PPP4R3A-001 - ENSP00000450864 [100%]
PPP4R3A-003 - ENSP00000450891 [100%]
PPP4R3A-007 - ENSP00000450883 [100%]
  PPP4R3A-001 - ENSP00000450864 [100%]
PPP4R3A-003 - ENSP00000450891 [100%]
PPP4R3A-007 - ENSP00000450883 [100%]
 

Other gene match

    
ANTIGEN VIEW
PPP4R3A-001
PPP4R3A-003
PPP4R3A-007
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.