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ANTIBODY INFORMATION
 
 

Antibody HPA051162

  

Antibody HPA053502

  

Antibody CAB018734

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA051162   HPA053502   sc-32879  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   12   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA051162

  

Antibody HPA053502

  

Antibody CAB018734

  
Immunocytochemistry


Validated

Validated

Validated
  

Validated

Validated
   Supported  
Immunohistochemistry

Supported   Supported  

Validated
  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA051162

  

Antibody HPA053502

  

Antibody CAB018734

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		      

 

Relative fluorescence intensity (RFI)

      

Figure description

 Cytoplasmic region of segmented cells in 10x-images      

Antibody dilution

 1:96      
 

Formal validation: Tagged protein
Validated

Antibody staining overlaps with GFP tagged protein		  
Validated

Antibody staining overlaps with GFP tagged protein		    

     

Figure description

 Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm, mitochondria & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.   Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & mitochondria and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.    

Antibody dilution

 1:96   1:200    
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA053502.		  
Validated

Antibody staining overlaps with antibody HPA051162.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.   Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.   Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.  

Antibody dilution

 1:96   1:200   1:188  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA051162

  

Antibody HPA053502

  

Antibody CAB018734

  

Formal validation: Orthogonal

    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 41 cell lines.  
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

   
 

 

  

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 51 cell types		   RNA: detected in 36 tissues
Protein: detected in 57 cell types		   RNA: detected in 36 tissues
Protein: detected in 19 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:900   1:2000   1:750  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA051162

  

Antibody HPA053502

  

Antibody CAB018734

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 70.7, 54.6, 47.5   70.7, 56, 52.5   70.7, 56, 54.6, 52.5, 47.5, 12.3, 11.9, 11.5  

Antibody dilution

 1:480   1:1130   1:500  

PROTEIN ARRAY
 
 

Antibody HPA051162

  

Antibody HPA053502

  

Antibody CAB018734

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:4000   1:30000    

ANTIGEN INFORMATION
 
 

Antibody HPA051162

  

Antibody HPA053502

  

Antibody CAB018734

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 92   81    

Antigen sequence

 
RLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEG
IHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKD
  
NARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPK
DFWEQEVRDIRSYLTEQVNQDLPKEVLAELE
  

											 
																				

																		

										
											
Matching transcripts

												
																					PCK2-001 - ENSP00000380171 [100%] 
PCK2-002 - ENSP00000216780 [100%] 
PCK2-003 - ENSP00000453444 [99%] 

											 
																						PCK2-002 - ENSP00000216780 [100%] 
PCK2-005 - ENSP00000454011 [100%] 
PCK2-007 - ENSP00000453656 [100%] 
PCK2-008 - ENSP00000441826 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PCK2-001
PCK2-002
PCK2-003
PCK2-005
PCK2-007
PCK2-008
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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