PCK2 - Antibodies - The Human Protein Atlas

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PCK2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA051162

Antibody HPA053502

Antibody CAB018734

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA051162

HPA053502

sc-32879

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA051162

Antibody HPA053502

Antibody CAB018734

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA051162

Antibody HPA053502

Antibody CAB018734

Formal validation: Genetic

Validated

Significant downregulation > 25% by one siRNA.

Relative fluorescence intensity (RFI)

Figure description

Cytoplasmic region of segmented cells in 10x-images

Antibody dilution

1:96

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm, mitochondria & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.

Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & mitochondria and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.

Antibody dilution

1:96

1:200

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA053502.

Validated

Antibody staining overlaps with antibody HPA051162.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.

Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.

Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.

Antibody dilution

1:96

1:200

1:188

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA051162

Antibody HPA053502

Antibody CAB018734

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 41 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Supported

Figure description

Immunohistochemical staining of human duodenum shows strong granular cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells of tubules.

Expression

RNA: detected in 36 tissues
Protein: detected in 51 cell types

RNA: detected in 36 tissues
Protein: detected in 57 cell types

RNA: detected in 36 tissues
Protein: detected in 19 cell types

Retrieval

HIER pH6

Antibody dilution

1:900

1:2000

1:750

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA051162

Antibody HPA053502

Antibody CAB018734

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

70.7, 54.6, 47.5

70.7, 56, 52.5

70.7, 56, 54.6, 52.5, 47.5, 12.3, 11.9, 11.5

Antibody dilution

1:480

1:1130

1:500

PROTEIN ARRAY

Antibody HPA051162

Antibody HPA053502

Antibody CAB018734

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:4000

1:30000

ANTIGEN INFORMATION

Antibody HPA051162

Antibody HPA053502

Antibody CAB018734

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

RLNWHGLSPLGWPSCRSIQTLRVLSGDLGQLPTGIRDFVEHSARLCQPEG
IHICDGTEAENTATLTLLEQQGLIRKLPKYNNCWLARTDPKD

NARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPK
DFWEQEVRDIRSYLTEQVNQDLPKEVLAELE

Matching transcripts

PCK2-001 - ENSP00000380171 [100%]
PCK2-002 - ENSP00000216780 [100%]
PCK2-003 - ENSP00000453444 [99%]

PCK2-002 - ENSP00000216780 [100%]
PCK2-005 - ENSP00000454011 [100%]
PCK2-007 - ENSP00000453656 [100%]
PCK2-008 - ENSP00000441826 [100%]

Other gene match

ANTIGEN VIEW

PCK2-001	PCK2-002	PCK2-003	PCK2-005	PCK2-007	PCK2-008

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.