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Immunohistochemical staining of human small intestine shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows nuclear positivity in glandular cells.
Expression
RNA: detected in 13 tissues Protein: detected in 52 cell types
RNA: detected in 13 tissues Protein: detected in 17 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:75
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004712
Antibody CAB019417
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400161)
Target mass (kDa)
52.8, 51.6, 50.1, 50, 49
52.8, 51.6, 50.1, 50, 49, 46.6, 44, 11.5
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA004712
Antibody CAB019417
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
