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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.
Antibody dilution
1:71
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA039392
Antibody HPA040503
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human testis shows strong nuclear and cytoplasmic positivity.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear staining in seminiferus ducts.
Expression
RNA: detected in 35 tissues Protein: detected in 75 cell types
RNA: detected in 35 tissues Protein: detected in 65 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:125
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039392
Antibody HPA040503
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
279.1, 228.4, 227
279.1, 228.4, 227
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039392
Antibody HPA040503
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.