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Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus & the Golgi apparatus.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Antibody dilution
1:125
1:77
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019550
Antibody HPA056051
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
Antibody dilution
1:150
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019550
Antibody HPA056051
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
96.6
96.6
Antibody dilution
1:250
1:260
PROTEIN ARRAY
Antibody HPA019550
Antibody HPA056051
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
