ZNF821 - Antibodies - The Human Protein Atlas

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ZNF821

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA036372

Antibody HPA042742

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036372

HPA042742

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA036372

Antibody HPA042742

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA036372

Antibody HPA042742

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.

Antibody dilution

1:85

1:200

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA036372

Antibody HPA042742

Standard validation

Approved

Figure description

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Expression

RNA: detected in 35 tissues
Protein: detected in 39 cell types

Retrieval

HIER pH6

Antibody dilution

1:350

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036372

Antibody HPA042742

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

46.8, 45.8, 42.1, 27.9, 23, 15.5

46.8, 45.8, 42.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA036372

Antibody HPA042742

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:6000

ANTIGEN INFORMATION

Antibody HPA036372

Antibody HPA042742

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

TNPNKVHCDSEGDEEETTQDEVSSHTSEEDGGVVKVEKELENTEQPVGGN
EVVEHEVTGNLNSDPLLELCQCPLCQ

LVCNNCAAYRKLLEAQTPSVRKWALRRQNEPLEVRLQRLERERTAKKSRR
DNETPEEREVRRMRDREAKRLQRMQETDEQRARRLQRDREAMRLKR

Matching transcripts

ZNF821-001 - ENSP00000405908 [100%]
ZNF821-018 - ENSP00000458112 [100%]
ZNF821-020 - ENSP00000456193 [100%]
ZNF821-201 - ENSP00000313822 [100%]
ZNF821-003 - ENSP00000456563 [89%]
ZNF821-006 - ENSP00000455648 [89%]
ZNF821-202 - ENSP00000398089 [89%]
ZNF821-203 - ENSP00000481362 [89%]
ZNF821-010 - ENSP00000455738 [86%]

ZNF821-001 - ENSP00000405908 [100%]
ZNF821-003 - ENSP00000456563 [100%]
ZNF821-006 - ENSP00000455648 [100%]
ZNF821-201 - ENSP00000313822 [100%]
ZNF821-202 - ENSP00000398089 [100%]

Other gene match

ANTIGEN VIEW

ZNF821-001	ZNF821-003	ZNF821-006	ZNF821-010	ZNF821-018	ZNF821-020	ZNF821-201	ZNF821-202	ZNF821-203

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.