RPGRIP1L - Antibodies - The Human Protein Atlas

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RPGRIP1L

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA039405

Antibody HPA040530

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA039405

HPA040530

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA039405

Antibody HPA040530

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA039405

Antibody HPA040530

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.

Antibody dilution

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA039405

Antibody HPA040530

Standard validation

Uncertain

Figure description

Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells.

Expression

RNA: detected in 33 tissues
Protein: detected in 46 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA039405

Antibody HPA040530

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

151.2, 147.3, 146.7, 144.8, 142.8

151.2, 147.3, 146.7, 144.8, 142.8, 41.3

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA039405

Antibody HPA040530

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

ANTIGEN INFORMATION

Antibody HPA039405

Antibody HPA040530

Antigen

Recombinant protein fragment

Length (aa)

115

109

Antigen sequence

KNTITLEVHQAYSTEYETIAACQLKFHEILEKSGRIFCTASLIGTKGDIP
NFGTVEYWFRLRVPMDQAIRLYRERAKALGYITSNFKGPEHMQSLSQQAP
KTAQLSSTDSTDGNL

TKYGNSLLEEARGEIRNLENVIQSQRGQIEELEHLAEILKTQLRRKENEI
ELSLLQLREQQATDQRSNIRDNVEMIKLHKQLVEKSNALSAMEGKFIQLQ
EKQRTLRIS

Matching transcripts

RPGRIP1L-001 - ENSP00000456534 [100%]
RPGRIP1L-003 - ENSP00000262135 [100%]
RPGRIP1L-007 - ENSP00000457889 [100%]
RPGRIP1L-009 - ENSP00000369257 [100%]
RPGRIP1L-201 - ENSP00000480698 [100%]

RPGRIP1L-001 - ENSP00000456534 [100%]
RPGRIP1L-003 - ENSP00000262135 [100%]
RPGRIP1L-005 - ENSP00000455295 [100%]
RPGRIP1L-007 - ENSP00000457889 [100%]
RPGRIP1L-009 - ENSP00000369257 [100%]
RPGRIP1L-201 - ENSP00000480698 [100%]

Other gene match

ANTIGEN VIEW

RPGRIP1L-001	RPGRIP1L-003	RPGRIP1L-005	RPGRIP1L-007	RPGRIP1L-009	RPGRIP1L-201

RELEVANT PUBLICATIONS

Antibody HPA039405

Antibody HPA040530

CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base
Wang WJ et al
Nat Cell Biol 2013;15(6):591-601

Superresolution Pattern Recognition Reveals the Architectural Map of the Ciliary Transition Zone
Yang TT et al
Sci Rep 2015;5:14096

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.