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BCKDK

ANTIBODY INFORMATION
 
 

Antibody HPA017995

  

Antibody HPA056067

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA017995   HPA056067  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA017995

  

Antibody HPA056067

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported    
Western blot

Supported   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA017995

  

Antibody HPA056067

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

   1:64  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA017995

  

Antibody HPA056067

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human salivary gland shows moderate cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 63 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:45    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA017995

  

Antibody HPA056067

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 46.4, 41.1, 37.8   46.4, 41.1  

Antibody dilution

 1:250   1:210  

PROTEIN ARRAY
 
 

Antibody HPA017995

  

Antibody HPA056067

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2550  

ANTIGEN INFORMATION
 
 

Antibody HPA017995

  

Antibody HPA056067

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 114   88  

Antigen sequence

 
LLDDHKDVVTLLAEGLRESRKHIEDEKLVRYFLDKTLTSRLGIRMLATHH
LALHEDKPDFVGIICTRLSPKKIIEKWVDFARRLCEHKYGNAPRVRINGH
VAARFPFIPMPLDY
  
TMESHLDTPYNVPDVVITIANNDVDLIIRISDRGGGIAHKDLDRVMDYHF
TTAEASTQDPRISPLFGHLDMHSGAQSGPMHGFGFGLP
 

Matching transcripts

 BCKDK-001 - ENSP00000219794 [100%]
BCKDK-002 - ENSP00000378404 [100%]
BCKDK-003 - ENSP00000287507 [100%]
BCKDK-004 - ENSP00000378405 [100%]
  BCKDK-001 - ENSP00000219794 [100%]
BCKDK-004 - ENSP00000378405 [100%]
BCKDK-002 - ENSP00000378404 [93%]
 

Other gene match

    
ANTIGEN VIEW
BCKDK-001
BCKDK-002
BCKDK-003
BCKDK-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.