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DNAJC17

ANTIBODY INFORMATION
 
 

Antibody HPA040914

  

Antibody HPA041187

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040914   HPA041187  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   8  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA040914

  

Antibody HPA041187

  
Immunocytochemistry

Approved    
Immunohistochemistry

Approved   Approved  
Western blot


Validated
   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040914

  

Antibody HPA041187

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:132    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040914

  

Antibody HPA041187

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human stomach, upper shows moderate cytoplasmic and nuclear positivity in glandular cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 48 cell types		   RNA: detected in 37 tissues
Protein: detected in 50 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:350   1:200  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA040914

  

Antibody HPA041187

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		    

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 34.7    

Loading control

 Total protein image    

Antibody dilution

 1:292    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 34.7   34.7, 15  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA040914

  

Antibody HPA041187

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA040914

  

Antibody HPA041187

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 83   67  

Antigen sequence

 
AVVEFATVKAAELAVQNEVGLVDNPLKISWLEGQPQDAVGRSHSGLSKGS
VLSERDYESLVMMRMRQAAERQQLIARMQQEDQ
  
NPRAAELFHQLSQALEVLTDAAARAAYDKVRKAKKQAAERTQKLDEKRKK
VKLDLEARERQAQAQES
 

Matching transcripts

 DNAJC17-001 - ENSP00000220496 [100%]
  DNAJC17-001 - ENSP00000220496 [100%]
DNAJC17-201 - ENSP00000485700 [100%]
 

Other gene match

    
ANTIGEN VIEW
DNAJC17-001
DNAJC17-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.