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OIP5

ANTIBODY INFORMATION
 
 

Antibody HPA052271

  

Antibody HPA059602

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA052271   HPA059602  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA052271

  

Antibody HPA059602

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA052271

  

Antibody HPA059602

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA059602.		  
Validated

Antibody staining overlaps with antibody HPA052271.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear speckles.  

Antibody dilution

 1:35   1:5  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA052271

  

Antibody HPA059602

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferous ducts and in Leydig cells.    

Expression

 RNA: detected in 28 tissues
Protein: detected in 33 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:100    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA052271

  

Antibody HPA059602

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 24.7   24.7, 17.4  

Antibody dilution

 1:180   1:80  

PROTEIN ARRAY
 
 

Antibody HPA052271

  

Antibody HPA059602

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1400   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA052271

  

Antibody HPA059602

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 85   109  

Antigen sequence

 
CGSCGIPVGFHLYSTHAALAALRGHFCLSSDKMVCYLLKTKAIVNASEMD
IQNVPLSEKIAELKEKIVLTHNRLKSLMKILSEVT
  
DFCGGTERAIDQASFTTSMEWDTQVVKGSSPLGPAGLGAEEPAAGPQLPS
WLQPERCAVFQCAQCHAVLADSVHLAWDLSRSLGAVVFSRVTNNVVLEAP
FLVGIEGSL
 

Matching transcripts

 OIP5-001 - ENSP00000220514 [100%]
  OIP5-001 - ENSP00000220514 [100%]
OIP5-002 - ENSP00000452851 [94%]
 

Other gene match

    
ANTIGEN VIEW
OIP5-001
OIP5-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.