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LAPTM4B

ANTIBODY INFORMATION
 
 

Antibody HPA068830

  

Antibody CAB020782

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA068830   3156.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 16   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA068830

  

Antibody CAB020782

  
Immunocytochemistry

Approved    
Immunohistochemistry

  Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA068830

  

Antibody CAB020782

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.    

Antibody dilution

 1:189    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA068830

  

Antibody CAB020782

  

Standard validation

   Uncertain  

   

Figure description

   Immunohistochemical staining of human colon shows strong positivity in glandular cells.  

Expression

   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:90  

Literature conformity

   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA068830

  

Antibody CAB020782

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 35.1, 25.4   35.1, 25.4, 17.3  

Antibody dilution

 1:420   1:500  

PROTEIN ARRAY
 
 

Antibody HPA068830

  

Antibody CAB020782

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:7500    

ANTIGEN INFORMATION
 
 

Antibody HPA068830

  

Antibody CAB020782

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 26    

Antigen sequence

 
PNSIQEYIRQLPPNFPYRDDVMSVNP
  

											 
																				

																		

										
											
Matching transcripts

												
																					LAPTM4B-001 - ENSP00000402301 [100%] 
LAPTM4B-002 - ENSP00000428409 [100%] 
LAPTM4B-201 - ENSP00000482533 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LAPTM4B-001
LAPTM4B-002
LAPTM4B-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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