SQLE - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

SQLE

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA018038

Antibody HPA020762

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA018038

HPA020762

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA018038

Antibody HPA020762

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA018038

Antibody HPA020762

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.

Antibody dilution

1:222

1:100

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA018038

Antibody HPA020762

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.

Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 53 cell types

RNA: detected in 36 tissues
Protein: detected in 66 cell types

Retrieval

HIER pH6

Antibody dilution

1:1000

1:400

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA018038

Antibody HPA020762

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

63.9, 53.1, 20.4

63.9, 53.1

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA018038

Antibody HPA020762

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA018038

Antibody HPA020762

Antigen

Recombinant protein fragment

Length (aa)

118

135

Antigen sequence

DGRKVTVIERDLKEPDRIVGEFLQPGGYHVLKDLGLGDTVEGLDAQVVNG
YMIHDQESKSEVQIPYPLSENNQVQSGRAFHHGRFIMSLRKAAMAEPNAK
FIEGVVLQLLEEDDVVMG

ELHAPLTVVADGLFSKFRKSLVSNKVSVSSHFVGFLMKNAPQFKANHAEL
ILANPSPVLIYQISSSETRVLVDIRGEMPRNLREYMVEKIYPQIPDHLKE
PFLEATDNSHLRSMPASFLPPSSVKKRGVLLLGDA

Matching transcripts

SQLE-001 - ENSP00000265896 [100%]
SQLE-002 - ENSP00000430331 [100%]
SQLE-004 - ENSP00000428239 [82%]

SQLE-001 - ENSP00000265896 [100%]
SQLE-002 - ENSP00000430331 [100%]

Other gene match

ANTIGEN VIEW

SQLE-001	SQLE-002	SQLE-004

RELEVANT PUBLICATIONS

Antibody HPA018038

Antibody HPA020762

Differential epigenetic reprogramming in response to specific endocrine therapies promotes cholesterol biosynthesis and cellular invasion
Nguyen VT et al
Nat Commun 2015;6:10044
Application: WB

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.