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MCM4

ANTIBODY INFORMATION
 
 

Antibody HPA004873

  

Antibody HPA031052

  

Antibody CAB004497

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA004873   HPA031052   sc-28317  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 3   12   3  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA004873

  

Antibody HPA031052

  

Antibody CAB004497

  
Immunocytochemistry


Validated

Validated
  

Validated

Validated
    
Immunohistochemistry


Validated
     Supported  
Western blot


Validated
  

Validated
   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA004873

  

Antibody HPA031052

  

Antibody CAB004497

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by one siRNA.		  
Validated

Significant downregulation > 25% by both siRNA:s.		    

 

Relative fluorescence intensity (RFI)

  
 

Relative fluorescence intensity (RFI)

    

Figure description

 Nuclear region of segmented cells in 10x-images   Nuclear region of segmented cells in 10x-images    

Antibody dilution

 1:10   1:101    
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA031052.		  
Validated

Antibody staining overlaps with antibody HPA004873.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.    

Antibody dilution

 1:18   1:101    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA004873

  

Antibody HPA031052

  

Antibody CAB004497

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		      

 

 

      

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 36 tissues.      
 

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human lymph node shows strong nuclear positivity in cells in reaction center.     Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells  

Expression

 RNA: detected in 37 tissues
Protein: detected in 48 cell types		     RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:50     1:100  

Literature conformity

 Consistent with gene/protein characterization data.     Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA004873

  

Antibody HPA031052

  

Antibody CAB004497

  

Formal validation: Genetic
Validated

Downregulation visible in both siRNA lanes		  
Validated

Downregulation visible in both siRNA lanes		    

     

Figure description

 Lane 1: Marker [kDa] 250, 150, 100, 75, 50, 37, 25, 20, 15, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		    

Target mass (kDa)

 96.6   96.6, 23.5    

Loading control

 Total protein image   Total protein image    

Antibody dilution

 1:100   1:224    
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 96.6   96.6, 23.5   96.6, 31.7, 23.5, 14.8  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA004873

  

Antibody HPA031052

  

Antibody CAB004497

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA004873

  

Antibody HPA031052

  

Antibody CAB004497

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 139   116    

Antigen sequence

 
LPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMA
VLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLE
SLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSAT
  
SSPPQMHSSAIPLDFDVSSPLTYGTPSSRVEGTPRSGVRGTPVRQRPDLG
SAQKGLQVDLQSDGAAAEDIVASEQSLGQKLVIWGTDVNVAACKENFQRF
LQRFIDPLAKEEENVG
  

											 
																				

																		

										
											
Matching transcripts

												
																					MCM4-001 - ENSP00000262105 [99%] 
MCM4-004 - ENSP00000430194 [99%] 

											 
																						MCM4-001 - ENSP00000262105 [100%] 
MCM4-004 - ENSP00000430194 [100%] 
MCM4-009 - ENSP00000428833 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MCM4-001
MCM4-004
MCM4-009
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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