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MAN2B1

ANTIBODY INFORMATION
 
 

Antibody HPA041530

  

Antibody HPA053404

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041530   HPA053404  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 8   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA041530

  

Antibody HPA053404

  
Immunocytochemistry

  Uncertain  
Immunohistochemistry

Supported    
Western blot

Uncertain   Supported  
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA041530

  

Antibody HPA053404

  

Standard validation

   Uncertain  

   

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.  

Antibody dilution

   1:44  

Literature conformity

   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041530

  

Antibody HPA053404

  

Standard validation

 Supported    

   

Figure description

 Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity with a granular pattern in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:125    

Literature conformity

 Consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA041530

  

Antibody HPA053404

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 113.7, 113.6   113.7, 113.6  

Antibody dilution

 1:250   1:220  

PROTEIN ARRAY
 
 

Antibody HPA041530

  

Antibody HPA053404

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:1750  

ANTIGEN INFORMATION
 
 

Antibody HPA041530

  

Antibody HPA053404

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 87   90  

Antigen sequence

 
TGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVATAQ
GRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVN
  
RHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRR
DYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDR
 

Matching transcripts

 MAN2B1-001 - ENSP00000395473 [100%]
MAN2B1-003 - ENSP00000221363 [100%]
  MAN2B1-001 - ENSP00000395473 [100%]
MAN2B1-003 - ENSP00000221363 [100%]
 

Other gene match

    
ANTIGEN VIEW
MAN2B1-001
MAN2B1-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.