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SLC17A7

ANTIBODY INFORMATION
 
 

Antibody HPA050458

  

Antibody HPA063679

  

Antibody CAB037183

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA050458   HPA063679   V0389  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   13   7  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA050458

  

Antibody HPA063679

  

Antibody CAB037183

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA050458

  

Antibody HPA063679

  

Antibody CAB037183

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebellum shows distinct cytoplasmic positivity in cells in granular layer and neuropil.   Immunohistochemical staining of human cerebral cortex shows positivity in neuropil.   Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in granular layer.  

Expression

 RNA: detected in 11 tissues
Protein: detected in 9 cell types		   RNA: detected in 11 tissues
Protein: detected in 10 cell types		   RNA: detected in 11 tissues
Protein: detected in 39 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:180   1:1350   1:1000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA050458

  

Antibody HPA063679

  

Antibody CAB037183

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 61.6   61.6, 53.9   61.6, 53.9  

Antibody dilution

 1:300   1:550   1:500  

PROTEIN ARRAY
 
 

Antibody HPA050458

  

Antibody HPA063679

  

Antibody CAB037183

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:6600    

ANTIGEN INFORMATION
 
 

Antibody HPA050458

  

Antibody HPA063679

  

Antibody CAB037183

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 22   31    

Antigen sequence

 
MEFRQEEFRKLAGRALGKLHRL
  
PSISEEERKYIEDAIGESAKLMNPLTKFSTP
  

											 
																				

																		

										
											
Matching transcripts

												
																					SLC17A7-001 - ENSP00000221485 [100%] 

											 
																						SLC17A7-001 - ENSP00000221485 [100%] 
SLC17A7-002 - ENSP00000470338 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SLC17A7-001
SLC17A7-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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