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ATP1A3

ANTIBODY INFORMATION
 
 

Antibody HPA045367

  

Antibody HPA056446

  

Antibody CAB033630

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA045367   HPA056446   sc-58631  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 13   13   13  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA045367

  

Antibody HPA056446

  

Antibody CAB033630

  
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045367

  

Antibody HPA056446

  

Antibody CAB033630

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in cells in molecular layer.   Immunohistochemical staining of human cerebellum shows strong positivity.   Immunohistochemical staining of human cerebral cortex shows distinctly stained neuropil.  

Expression

 RNA: detected in 10 tissues
Protein: detected in 18 cell types		   RNA: detected in 10 tissues
Protein: detected in 27 cell types		   RNA: detected in 10 tissues
Protein: detected in 6 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:1200   1:5000  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045367

  

Antibody HPA056446

  

Antibody CAB033630

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

      

Target mass (kDa)

 113.1, 111.7   113.1, 111.7, 108.3, 27.3   113.1, 111.7, 108.3, 27.3  

Antibody dilution

 1:250   1:120   1:500  

PROTEIN ARRAY
 
 

Antibody HPA045367

  

Antibody HPA056446

  

Antibody CAB033630

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1300    

ANTIGEN INFORMATION
 
 

Antibody HPA045367

  

Antibody HPA056446

  

Antibody CAB033630

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 26   33    

Antigen sequence

 
DKKDDKDSPKKNKGKERRDLDDLKKE
  
EVAMTEHKMSVEEVCRKYNTDCVQGLTHSKAQE
  

											 
																				

																		

										
											
Matching transcripts

												
																					ATP1A3-001 - ENSP00000302397 [100%] 
ATP1A3-007 - ENSP00000437577 [100%] 
ATP1A3-008 - ENSP00000444688 [100%] 

											 
																						ATP1A3-001 - ENSP00000302397 [100%] 
ATP1A3-007 - ENSP00000437577 [100%] 
ATP1A3-008 - ENSP00000444688 [100%] 
ATP1A3-005 - ENSP00000471581 [91%] 
ATP1A3-011 - ENSP00000469129 [91%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ATP1A3-001
ATP1A3-005
ATP1A3-007
ATP1A3-008
ATP1A3-011
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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