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Immunohistochemical staining of human spleen shows moderate cytoplasmic positivity in cells in red pulp and white pulp. Red blood cells were distinctly stained.
Expression
RNA: detected in 1 tissues Protein: detected in 6 cell types
Retrieval
HIER pH6
Antibody dilution
1:650
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA061286
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
22.3
Antibody dilution
1:610
PROTEIN ARRAY
Antibody HPA061286
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
