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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & nuclear membrane.
Antibody dilution
1:81
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA034958
Antibody HPA058534
Standard validation
Approved
Figure description
Immunohistochemical staining of human liver shows strong nuclear positivity in hepatocytes.
Expression
RNA: detected in 33 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
Antibody dilution
1:250
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034958
Antibody HPA058534
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
28.3, 27.2
28.3, 27.2
Antibody dilution
1:250
1:270
PROTEIN ARRAY
Antibody HPA034958
Antibody HPA058534
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
