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Immunohistochemical staining of human fallopian tube shows strong positivity in cilia of glandular cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in endocrine cells.
Expression
RNA: detected in 16 tissues Protein: detected in 35 cell types
RNA: detected in 16 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:225
1:120
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA056968
Antibody HPA057155
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
52.8, 50.8, 45.1
52.8, 50.8, 45.1
Antibody dilution
1:160
1:130
PROTEIN ARRAY
Antibody HPA056968
Antibody HPA057155
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.