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GRB10

ANTIBODY INFORMATION
 
 

Antibody HPA027502

  

Antibody HPA031818

  

Antibody CAB019423

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Cell Signaling Technology, Inc
  

Product name

 HPA027502   HPA031818   3702  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 6   13   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA027502

  

Antibody HPA031818

  

Antibody CAB019423

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Approved     Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA027502

  

Antibody HPA031818

  

Antibody CAB019423

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line A549 shows localization to nuclear membrane & vesicles.    

Antibody dilution

   1:30    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027502

  

Antibody HPA031818

  

Antibody CAB019423

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules.     Immunohistochemical staining of human rectum shows strong granular cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 48 cell types		     RNA: detected in 37 tissues
Protein: detected in 68 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:25     1:40  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027502

  

Antibody HPA031818

  

Antibody CAB019423

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 67.2, 66.5, 62, 60.8   67.2, 66.5, 62, 12.4   67.2, 66.5, 62, 60.8, 12.7, 12.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA027502

  

Antibody HPA031818

  

Antibody CAB019423

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA027502

  

Antibody HPA031818

  

Antibody CAB019423

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 79   94    

Antigen sequence

 
FSGQTGRVIENPAEAQSAALEEGHAWRKRSTRMNILGSQSPLHPSTLSTV
IHRTQHWFHGRISREESHRIIKQQGLVDG
  
LHHPYYQDKVEQTPRSQQDPAGPGLPAQSDRLANHQEDDVDLEALVNDMN
ASLESLYSACSMQSDTVPLLQNGQHARSQPRASGPPRSIQPQVS
  

											 
																				

																		

										
											
Matching transcripts

												
																					GRB10-001 - ENSP00000385544 [100%] 
GRB10-002 - ENSP00000385748 [100%] 
GRB10-003 - ENSP00000385770 [100%] 
GRB10-004 - ENSP00000385366 [100%] 
GRB10-005 - ENSP00000349818 [100%] 
GRB10-006 - ENSP00000385189 [100%] 
GRB10-007 - ENSP00000385046 [100%] 
GRB10-201 - ENSP00000338543 [100%] 
GRB10-202 - ENSP00000381790 [100%] 
GRB10-203 - ENSP00000381793 [100%] 

											 
																						GRB10-003 - ENSP00000385770 [100%] 
GRB10-005 - ENSP00000349818 [100%] 
GRB10-203 - ENSP00000381793 [100%] 
GRB10-001 - ENSP00000385544 [93%] 
GRB10-014 - ENSP00000413023 [93%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

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GRB10-001
GRB10-002
GRB10-003
GRB10-004
GRB10-005
GRB10-006
GRB10-007
GRB10-014
GRB10-201
GRB10-202
GRB10-203
								

							

						

							
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