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Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & cell junctions.
Antibody dilution
1:43
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019033
Antibody HPA019167
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic and membranous positivity in urothelial cells.
Expression
RNA: detected in 33 tissues Protein: detected in 71 cell types
RNA: detected in 33 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:50
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019033
Antibody HPA019167
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
136.7, 135.6, 122.5, 119
136.7, 135.6, 119, 66.3, 51.2, 43.3, 41.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA019033
Antibody HPA019167
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
