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Immunofluorescent staining of human cell line A549 shows localization to cytosol.
Antibody dilution
1:95
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019098
Antibody HPA020057
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA020057 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA019098 across 46 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Expression
RNA: detected in 37 tissues Protein: detected in 66 cell types
RNA: detected in 37 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:200
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019098
Antibody HPA020057
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416745)
Target mass (kDa)
35.3
35.3, 27.8, 26.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA019098
Antibody HPA020057
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
