AHR - Antibodies - The Human Protein Atlas

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AHR

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA029722

Antibody HPA029723

Antibody CAB005072

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA029722

HPA029723

sc-5579

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA029722

Antibody HPA029723

Antibody CAB005072

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA029722

Antibody HPA029723

Antibody CAB005072

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

Antibody dilution

1:200

1:19

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA029722

Antibody HPA029723

Antibody CAB005072

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows moderate cytoplasmic and nuclear positivity in neuronal cells.

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of glial cells.

Immunohistochemical staining of human lymph node shows cytoplasmic positivity in reaction center cells.

Expression

RNA: detected in 36 tissues
Protein: detected in 62 cell types

RNA: detected in 36 tissues
Protein: detected in 54 cell types

RNA: detected in 36 tissues
Protein: detected in 66 cell types

Retrieval

HIER pH6

Antibody dilution

1:500

1:75

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA029722

Antibody HPA029723

Antibody CAB005072

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

96.1

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA029722

Antibody HPA029723

Antibody CAB005072

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA029722

Antibody HPA029723

Antibody CAB005072

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

NWQDNTAPMGNDTILKHEQIDQPQDVNSFAGGHPGLFQDSKNSDLYSIMK
NLGIDFEDIRHMQNEKFFRNDFSGEVDFRDIDLTDEI

PQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQ
NGVLNETYPAELNNINNTQTTTHLQPLHHPSE

Matching transcripts

AHR-002 - ENSP00000242057 [100%]

Other gene match

ANTIGEN VIEW

AHR-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.