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Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Expression
RNA: detected in 9 tissues Protein: detected in 4 cell types
RNA: detected in 9 tissues Protein: detected in 24 cell types
RNA: detected in 9 tissues Protein: detected in 28 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:90
1:150
1:80
Literature conformity
Consistent with gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA013331
Antibody HPA039262
Antibody CAB033884
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412006)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400153)