Fields »
ABOUT
HELP
BLOG
SPIN1

ANTIBODY INFORMATION
 
 

Antibody HPA000162

  

Antibody HPA068784

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000162   HPA068784  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 SPIN2A - ENSG00000147059 [99%]
SPIN2B - ENSG00000186787 [100%]
    

Released in version

 1   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA000162

  

Antibody HPA068784

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000162

  

Antibody HPA068784

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.  

Antibody dilution

   1:74  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000162

  

Antibody HPA068784

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 58 cell types		    

Retrieval

 None    

Antibody dilution

 1:50    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA000162

  

Antibody HPA068784

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 29.6, 29.2, 27.8   29.6  

Antibody dilution

 1:500   1:250  

PROTEIN ARRAY
 
 

Antibody HPA000162

  

Antibody HPA068784

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2950  

ANTIGEN INFORMATION
 
 

Antibody HPA000162

  

Antibody HPA068784

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 107   53  

Antigen sequence

 
AVEHMFEGEHGSKDEWRGMVLAQAPIMKAWFYITYEKDPVLYMYQLLDDY
KEGDLRIMPESSESPPTEREPGGVVDGLIGKHVEYTKEDGSKRIGMVIHQ
VEAKPSV
  
MKTPFGKTPGQRSRADAGHAGVSANMMKKRTSHKKHRSSVGPSKPVSQPR
RNI
 

Matching transcripts

 SPIN1-001 - ENSP00000365019 [85%]
  SPIN1-001 - ENSP00000365019 [100%]
 

Other gene match

 SPIN2A - ENSG00000147059 [99%]
SPIN2B - ENSG00000186787 [100%]
    
ANTIGEN VIEW
SPIN1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.