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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Antibody dilution
1:23
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA008435
Antibody HPA024230
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human smooth muscle shows distinctly positivity in extracellular matrix.
Immunohistochemical staining of human bronchus shows strong nuclear positivity in respiratory epithelial cells and distinct staining in extra cellular material.
Expression
RNA: detected in 35 tissues Protein: detected in 10 cell types
RNA: detected in 35 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:35
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008435
Antibody HPA024230
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
43.4
43.4, 27.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA008435
Antibody HPA024230
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
