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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane.
Antibody dilution
1:200
1:25
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA019713
Antibody CAB018587
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells.
Expression
RNA: detected in 35 tissues Protein: detected in 51 cell types
RNA: detected in 35 tissues Protein: detected in 24 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:800
1:100
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019713
Antibody CAB018587
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY402303)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
21.9, 19.7
21.9, 19.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA019713
Antibody CAB018587
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
