EIF3A - Antibodies - The Human Protein Atlas

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EIF3A

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA038315

Antibody HPA038316

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA038315

HPA038316

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA038315

Antibody HPA038316

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA038315

Antibody HPA038316

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cytosol.

Antibody dilution

1:44

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA038315

Antibody HPA038316

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA038316 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA038315 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 67 cell types

RNA: detected in 37 tissues
Protein: detected in 72 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:35

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA038315

Antibody HPA038316

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

166.6

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA038315

Antibody HPA038316

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA038315

Antibody HPA038316

Antigen

Recombinant protein fragment

Length (aa)

103

Antigen sequence

LQNNTILRLLQQVSQIYQSIEFSRLTSLVPFVDAFQLERAIVDAARHCDL
QVRIDHTSRTLSFGSDLNYATREDAPIG

PENALKRANEFLEVGKKQPALDVLYDVMKSKKHRTWQKIHEPIMLKYLEL
CVDLRKSHLAKEGLYQYKNICQQVNIKSLEDVVRAYLKMAEEKTEAAKEE
SQQ

Matching transcripts

EIF3A-001 - ENSP00000358140 [100%]
EIF3A-201 - ENSP00000438178 [100%]

Other gene match

ANTIGEN VIEW

EIF3A-001	EIF3A-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.