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ARHGAP21

ANTIBODY INFORMATION
 
 

Antibody HPA036610

  

Antibody HPA057861

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036610   HPA057861  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA036610

  

Antibody HPA057861

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036610

  

Antibody HPA057861

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line HeLa shows localization to plasma membrane & actin filaments.  

Antibody dilution

   1:71  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036610

  

Antibody HPA057861

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human gall bladder shows distinct nuclear and cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 59 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:200    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA036610

  

Antibody HPA057861

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 217.5, 129.4, 125.6   217.5, 156.2, 156  

Antibody dilution

 1:250   1:240  

PROTEIN ARRAY
 
 

Antibody HPA036610

  

Antibody HPA057861

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:2850  

ANTIGEN INFORMATION
 
 

Antibody HPA036610

  

Antibody HPA057861

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 96   88  

Antigen sequence

 
VPPCLTTSAPLIRRQLSHDHESVGPPSLDAQPNSKTERSKSYDEGLDDYR
EDAKLSFKHVSSLKGIKIADSQKSSEDSGSRKDSSSEVFSDAAKEG
  
QEPTWKTKIADRLKLRPRAPADDMFGVGNHKVNAETAKRKSIRRRHTLGG
HRDATEISVLNFWKVHEQSGERESELSAVNRLKPKCSA
 

Matching transcripts

 ARHGAP21-001 - ENSP00000379709 [100%]
ARHGAP21-003 - ENSP00000405018 [100%]
ARHGAP21-012 - ENSP00000365592 [100%]
  ARHGAP21-001 - ENSP00000379709 [100%]
ARHGAP21-201 - ENSP00000365604 [100%]
ARHGAP21-202 - ENSP00000480005 [100%]
 

Other gene match

    
ANTIGEN VIEW
ARHGAP21-001
ARHGAP21-003
ARHGAP21-012
ARHGAP21-201
ARHGAP21-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.