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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus & nucleoli fibrillar center.
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Antibody dilution
1:34
1:25
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
WESTERN BLOT
Antibody HPA037924
Antibody HPA037925
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410967)
Target mass (kDa)
42.1
42.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA037924
Antibody HPA037925
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
