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Antibody staining overlaps with antibody HPA061547.
Validated
Antibody staining overlaps with antibody HPA038043.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus.
Antibody dilution
1:26
1:39
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038042
Antibody HPA038043
Antibody HPA061547
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human liver shows moderate membranous positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 11 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:150
1:50
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038042
Antibody HPA038043
Antibody HPA061547
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Target too small/large to be analyzed with the present setup.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
268.8
268.8
268.8
Antibody dilution
1:250
1:250
1:130
PROTEIN ARRAY
Antibody HPA038042
Antibody HPA038043
Antibody HPA061547
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
