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SMC3

ANTIBODY INFORMATION
 
 

Antibody HPA037411

  

Antibody HPA043206

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA037411   HPA043206  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA037411

  

Antibody HPA043206

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Uncertain    
Western blot

Uncertain  

Validated
  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA037411

  

Antibody HPA043206

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

   1:6  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA037411

  

Antibody HPA043206

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 66 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:25    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA037411

  

Antibody HPA043206

  

Formal validation: Genetic

  
Validated

Downregulation visible in one of two siRNA lanes		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

   141.5  

Loading control

   Total protein image  

Antibody dilution

   1:58  
 

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 141.5   141.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA037411

  

Antibody HPA043206

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA037411

  

Antibody HPA043206

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 88   85  

Antigen sequence

 
EFNKMNLPGEVTFLPLNKLDVRDTAYPETNDAIPMISKLRYNPRFDKAFK
HVFGKTLICRSMEVSTQLARAFTMDCITLEGDQVSHRG
  
ALLHEGTGPRVISAFVEIIFDNSDNRLPIDKEEVSLRRVIGAKKDQYFLD
KKMVTKNDVMNLLESAGFSRSNPYYIVKQGKINQM
 

Matching transcripts

 SMC3-001 - ENSP00000354720 [100%]
  SMC3-001 - ENSP00000354720 [100%]
 

Other gene match

    
ANTIGEN VIEW
SMC3-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.