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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Antibody dilution
1:6
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA021587
Antibody HPA022905
Antibody CAB006855
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human cerebellum shows moderate cytoplasmic positivity in cells in granular layer and distinctly stained neuropil.
Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in purkinje cells and cells in molecular layer.
Immunohistochemical staining of human pancreas shows distinct cytoplasmic positivity in exocrine glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 14 cell types
RNA: detected in 36 tissues Protein: detected in 23 cell types
RNA: detected in 36 tissues Protein: detected in 75 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:35
1:30
1:300
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA021587
Antibody HPA022905
Antibody CAB006855
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil