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ALOX12

ANTIBODY INFORMATION
 
 

Antibody HPA010691

  

Antibody CAB019287

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA010691   2786.00.02  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 4   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA010691

  

Antibody CAB019287

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA010691

  

Antibody CAB019287

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & cytosol.  

Antibody dilution

   1:56  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA010691

  

Antibody CAB019287

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human heart muscle shows distinct positivity in myocytes.   Immunohistochemical staining of human smooth muscle shows distinct positivity in smooth muscle cells.  

Expression

 RNA: detected in 32 tissues
Protein: detected in 70 cell types		   RNA: detected in 32 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:225  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA010691

  

Antibody CAB019287

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 75.7   75.7, 16.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA010691

  

Antibody CAB019287

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA010691

  

Antibody CAB019287

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 101    

Antigen sequence

 
YAWVPNAPCTMRMPPPTTKEDVTMATVMGSLPDVRQACLQMAISWHLSRR
QPDMVPLGHHKEKYFSGPKPKAVLNQFRTDLEKLEKEITARNEQLDWPYE
Y
  

											 
																				

																		

										
											
Matching transcripts

												
																					ALOX12-001 - ENSP00000251535 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
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