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Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:28
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA054459
Antibody HPA066328
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human heart muscle shows strong membranous positivity in myocytes.
Expression
RNA: detected in 28 tissues Protein: detected in 24 cell types
Retrieval
HIER pH6
Antibody dilution
1:175
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054459
Antibody HPA066328
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
57.5, 57.4
57.5, 57.4, 14.4, 9.5
Antibody dilution
1:150
1:90
PROTEIN ARRAY
Antibody HPA054459
Antibody HPA066328
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
