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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli.
Antibody dilution
1:32
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA013333
Antibody HPA014641
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human smooth muscle shows modrate cytoplamsic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows weak cytoplasmic positivity in smooth muscle cells.
Expression
RNA: detected in 37 tissues Protein: detected in 25 cell types
RNA: detected in 37 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:150
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA013333
Antibody HPA014641
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412070)
Target mass (kDa)
36.2
36.2, 24.7
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA013333
Antibody HPA014641
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
