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ANTIBODY INFORMATION
 
 

Antibody HPA008720

  

Antibody CAB023519

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   AbD Serotec
  

Product name

 HPA008720   0300-0007  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 4   5  

References

 1    

VALIDATION SUMMARY
 
 

Antibody HPA008720

  

Antibody CAB023519

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008720

  

Antibody CAB023519

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.  

Antibody dilution

   1:200  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008720

  

Antibody CAB023519

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human placenta shows strong membranous positivity in cytotrophoblastic cells.    

Expression

 RNA: detected in 30 tissues
Protein: detected in 31 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:1000    

Literature conformity

 Partly consistent with extensive gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA008720

  

Antibody CAB023519

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 30.3, 27.9   30.3, 27.9  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008720

  

Antibody CAB023519

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA008720

  

Antibody CAB023519

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 142    

Antigen sequence

 
FSGDHSADGFEVTSRSEMSSGSEISPVSEMPSSSEPSSGADYDYSEEYDN
EPQIPGYIVDDSVRVEQVVKPPQNKTESENTSDKPKRKKKGGKNGKNRRN
RKKKNPCNAEFQNFCIHGECKYIEHLEAVTCKCQQEYFGERC
  

											 
																				

																		

										
											
Matching transcripts

												
																					AREG-001 - ENSP00000379097 [100%] 
AREG-002 - ENSP00000421414 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
AREG-001
AREG-002
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008720

												
										 
																				
											
											
											
Antibody CAB023519

												
										 
																		

																	

										
											
1.

												
																						Multiple molecular subtypes of triple-negative breast cancer critically rely on androgen receptor and respond to enzalutamide in vivo

	Barton VN et al

	Mol Cancer Ther 2015;14(3):769-78

			Application: IHC				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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