WHSC1 - Antibodies - The Human Protein Atlas

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WHSC1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA015801

Antibody CAB068246

Antibody CAB068247

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA015801

AMAb90851

AMAb90848

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA015801

Antibody CAB068246

Antibody CAB068247

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA015801

Antibody CAB068246

Antibody CAB068247

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:49

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA015801

Antibody CAB068246

Antibody CAB068247

Standard validation

Supported

Figure description

Immunohistochemical staining of human oral mucosa shows strong nuclear positivity in squamous epithelial cells.

Immunohistochemical staining of human lymph node shows nuclear positivity in germinal center cells.

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal cells, glial cells were moderately stained.

Expression

RNA: detected in 37 tissues
Protein: detected in 73 cell types

RNA: detected in 37 tissues
Protein: detected in 67 cell types

RNA: detected in 37 tissues
Protein: detected in 73 cell types

Retrieval

HIER pH6

Antibody dilution

1:40

1:2000

1:200

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA015801

Antibody CAB068246

Antibody CAB068247

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

152.3, 71.4, 69.4, 43.2, 30.2, 21.7

152.3, 80.8, 71.4, 69.4, 43.2, 31.2, 30.2, 21.7, 4

Antibody dilution

1:250

1:1000

PROTEIN ARRAY

Antibody HPA015801

Antibody CAB068246

Antibody CAB068247

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA015801

Antibody CAB068246

Antibody CAB068247

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

117

Antigen sequence

SANGKTPSCEVNRECSVFLSKAQLSSSLQEGVMQKFNGHDALPFIPADKL
KDLTSRVFNGEPGAHDAKLRFESQEMKGIGTPPNTTPIKNGSPEIKLKIT
KTYMNGKPLFESSICGD

Matching transcripts

WHSC1-004 - ENSP00000425761 [100%]
WHSC1-006 - ENSP00000399251 [100%]
WHSC1-008 - ENSP00000372351 [100%]
WHSC1-010 - ENSP00000421681 [100%]
WHSC1-011 - ENSP00000423972 [100%]
WHSC1-014 - ENSP00000427434 [100%]
WHSC1-015 - ENSP00000422878 [100%]
WHSC1-201 - ENSP00000372347 [100%]
WHSC1-202 - ENSP00000372348 [100%]
WHSC1-203 - ENSP00000381311 [100%]
WHSC1-204 - ENSP00000416725 [100%]

Other gene match

ANTIGEN VIEW

WHSC1-004	WHSC1-006	WHSC1-008	WHSC1-010	WHSC1-011	WHSC1-014	WHSC1-015	WHSC1-201	WHSC1-202	WHSC1-203	WHSC1-204

RELEVANT PUBLICATIONS

Antibody HPA015801

Antibody CAB068246

Antibody CAB068247

Histone lysine methyltransferase Wolf-Hirschhorn syndrome candidate 1 is involved in human carcinogenesis through regulation of the Wnt pathway
Toyokawa G et al
Neoplasia 2011;13(10):887-98

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.