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Immunofluorescent staining of human cell line RT4 shows localization to nucleus.
Antibody dilution
1:149
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA055004
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous staining in trophoblastic cells, while strong cytoplasmic positivity was observed in decidual cells.
Expression
RNA: detected in 36 tissues Protein: detected in 50 cell types
Retrieval
HIER pH6
Antibody dilution
1:700
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055004
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
82.7, 53.2, 43.8
Antibody dilution
1:750
PROTEIN ARRAY
Antibody HPA055004
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
