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Antibody staining overlaps with antibody HPA040029.
Validated
Antibody staining overlaps with antibody HPA039485.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Antibody dilution
1:57
1:41
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA039485
Antibody HPA040029
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA040029 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA039485 across 72 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 42 cell types
RNA: detected in 37 tissues Protein: detected in 42 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:50
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039485
Antibody HPA040029
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414641)
Target mass (kDa)
110.3
110.3
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA039485
Antibody HPA040029
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
