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Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in umbrella cells.
Immunohistochemical staining of human urinary bladder shows strong membranous positivity in urothelial cells.
Expression
RNA: detected in 3 tissues Protein: detected in 17 cell types
RNA: detected in 3 tissues Protein: detected in 9 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:1500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043312
Antibody HPA061106
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
19.4
19.4
Antibody dilution
1:250
1:230
PROTEIN ARRAY
Antibody HPA043312
Antibody HPA061106
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
