PDHX - Antibodies - The Human Protein Atlas

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PDHX

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA038484

Antibody HPA038485

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA038484

HPA038485

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA038484

Antibody HPA038485

Immunocytochemistry

Uncertain

Immunohistochemistry

Western blot

Supported

Protein array

Approved

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA038484

Antibody HPA038485

Standard validation

Uncertain

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.

Antibody dilution

1:127

1:135

Literature conformity

The subcellular location is not consistent with literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA038484

Antibody HPA038485

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA038485 across 70 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA038484 across 70 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic(granular pattern) and nuclear positivity in glandular cells.

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 73 cell types

RNA: detected in 37 tissues
Protein: detected in 66 cell types

Retrieval

HIER pH6

Antibody dilution

1:1200

1:1000

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA038484

Antibody HPA038485

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

54.1, 51.5, 19.7, 16.2

54.1, 51.5

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA038484

Antibody HPA038485

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:6000

ANTIGEN INFORMATION

Antibody HPA038484

Antibody HPA038485

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

DASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPP
VSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLS

PAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTP
APTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEI

Matching transcripts

PDHX-001 - ENSP00000227868 [100%]
PDHX-002 - ENSP00000389404 [100%]
PDHX-003 - ENSP00000431281 [100%]
PDHX-008 - ENSP00000432277 [100%]

PDHX-001 - ENSP00000227868 [100%]
PDHX-002 - ENSP00000389404 [100%]

Other gene match

ANTIGEN VIEW

PDHX-001	PDHX-002	PDHX-003	PDHX-008

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.