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Immunofluorescent staining of human cell line A-431 shows localization to nucleus & cytosol.
Antibody dilution
1:111
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA042395
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in respiratory epithelial cells with distinct extracellular material.
Expression
RNA: detected in 33 tissues Protein: detected in 53 cell types
Retrieval
HIER pH6
Antibody dilution
1:200
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042395
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
100, 96.5, 43.2, 16.9
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA042395
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
