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Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Antibody dilution
1:200
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031608
Antibody HPA031609
Antibody HPA031610
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA031609 across 76 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA031608 across 76 cell types.
Standard validation
Approved
Approved
Approved
Figure description
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 70 cell types
RNA: detected in 37 tissues Protein: detected in 67 cell types
RNA: detected in 37 tissues Protein: detected in 78 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:100
1:150
1:25
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031608
Antibody HPA031609
Antibody HPA031610
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
251.5, 83.7, 19.8
251.5, 83.7
251.5
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA031608
Antibody HPA031609
Antibody HPA031610
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:3000
1:3000
ANTIGEN INFORMATION
Antibody HPA031608
Antibody HPA031609
Antibody HPA031610
Antigen
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
92
90
Antigen sequence
GREAIESGAAFLFMTFHLKDSVGHKETKAIKQMFGPFPSSSATAACNATN
RIISHFSQDDLTALVQMTEKEHGDRVFFGKNLAFSFDMHDLDHFDELPIN
G
